It is not immediately clear how the animal escaped.
Officials said he was spotted by a staff member roaming freely inside Audubon Zoo, in New Orleans, around 7.20am yesterday morning. The big cat went on a rampage before he was sedated at about 8.15am.
NOLA.com reports the fifth alpaca died overnight after the male jaguar killed four alpacas, one emu and one fox early Saturday.
A JAGUAR escaped from its zoo enclosure and slaughtered seven animals in a terrifying hour-long killing spree in the US.
Another alpaca and a second fox later died from their injuries taking the total number of animals killed by the jaguar to eight.
No people were injured during the escape, and the zoo remained closed on Saturday.
"We are certainly not going to euthanize the animal over this", said Joel Hamilton, vice president and general curator for the zoo. The zoo will also be working with a child psychologist for Zoo campers so they can assist with helping parents and children cope with the incident.
No explanation has been given for the jaguar's escape, but the zoo said an "after-action review" is taking place, as well as an investigation into how the jaguar escaped.
Despite sympathy for the lost animals, many online couldn't blame Valerio for doing what jaguars do.
In a Saturday afternoon update, zoo officials said their initial inspection suggested the roof of the jaguar habitat was "compromised". The other is a female, named Ix Chel, who was in a separate indoor enclosure when Valerio broke out.
There will be grief counselors at the zoo Sunday morning for staff.