US Defence Secretary James Mattis said here that Washington was committed to "holding accountable" those responsible for killing Saudi Arabian journalist Jamal Khashoggi, whose death "undermines regional stability".
And while Mattis said the failure of any nation to adhere to worldwide norms and rule of law "undermines regional stability at a time when it is needed most", he also stressed the strength of Saudi-US relations by saying "We maintain our strong people-to-people partnership knowing that with our respect must come transparency and trust as indicated by President Trump, Secretary Pompeo and European leaders alike".
The minister also said the administration of US President Donald Trump has a "rational, realistic" foreign policy that all Gulf Arab states can support.
Saudi state television quoted the Saudi public prosecutor on Thursday as saying Riyadh was interrogating suspects on the basis of information given by a joint Saudi-Turkish task force.
The Committee to Protect Journalists, meanwhile, has called for "an worldwide investigation and relentless pressure on Saudi Arabia from the Trump administration, if we ever hope to get to the truth".
Mr Trump also said Riyadh had staged the "worst cover-up ever" over the killing.
Turkish police were investigating water samples from a well at the consulate on Thursday after initially being denied access, broadcaster CNN Turk said.
Trudeau said he might cut off any arms export permits that allow Canadian manufacturers of military equipment to ship their goods to Saudi Arabia.
Khashoggi, who lived in self-imposed exile in the United States for almost a year before his death, had written critically of Prince Mohammed's crackdown on dissent.
Slain Saudi journalist Jamal Khashoggi assumed authorities would not give him problems or arrest him in Turkey, though he was concerned tensions would arise when he visited the consulate in Istanbul, his fiancee said on Friday. Prince Mohammed, Riyadh's de facto ruler who casts himself as a reformer, has said the killers will be brought to justice.
US Secretary of State Mike Pompeo this week announced moves against 21 Saudis to either revoke their visas or make them ineligible for US visas after the Khashoggi killing.
Media captionJamal Khashoggi: What we know about the journalist's disappearance and death What else did his fiancée say?
The ministry referred the extradition request for the suspects - all of whom are Saudi nationals - to Turkish Foreign Ministry, the statement said.
While these were some of the sharpest comments Mattis has made on the Khashoggi killing, he said the incident would not diminish ties with Saudi Arabia.
"Even Saudi Arabia itself has admitted that the crime was premeditated and involved state officials", Agnes Callamard, United Nations Special Rapporteur on Extrajudicial, Summary or Arbitrary Executions, said during a press conference.
Al-Jubeir addressed the Khashoggi case in a question-and-answer session, in which several reporters asked different questions about Saudi Arabia's handling of the case.